The In-Cell WesternTM (ICW) Assay is a quantitative immunofluorescence assay that combines Western blotting with high-throughput ELISA. Using this technique, researchers measure protein levels in cells grown in microplates in less time than it takes to run a Western blot. The ICW Assay does not require researchers to create cell lysates or run samples on a gel, which saves time and preserves the proteins’ cellular context. Scientists use the ICW Assay in a variety of ways, such as for analyzing protein phosphorylation, viral titer, RNAi efficacy, gene expression, and apoptosis.
Download this handbook from LI-COR Biosciences to learn how to develop In-Cell Western Assays, from start to finish.
- Cell culture
- Antibody selection
- Signal normalization
- Fixation, permeabilization, and buffers
- Cell staining