Immune cells are constantly changing and adapting their properties and functions based on external cues from the local environment or from interactions with other cells. As such, information on spatial context is critical to accurately profile cells and understand their behavior. However, researchers trying to characterize cell populations often have to choose between techniques like flow cytometry that simultaneously screen many parameters but do not retain spatial information, or techniques like immunohistochemistry that offer spatial context but can only interrogate a few markers at a time.

ChipCytometry is a chip-based immunoassay that offers multiplexing capacity while retaining spatial information. Through repeated, rapid stain-image-photobleach cycles, researchers probe a theoretically infinite number of markers per sample, five probes at a time. Chips store samples for up to two years, allowing researchers to build on prior findings.

Download this ebook from Canopy Biosciences to learn

  • The importance of spatial biology to cellular function
  • How ChipCytometry works
  • The ChipCytometry workflow
  • How ChipCytometry is contributing to new discoveries in immunological research